PLMD Protein Information


Tag Content
PLMD ID PLMD-0002977
Uniprot Accession P02340; Q9QUP3
Genbank Protein ID X00876; X00877; X00878; X00879; X00880; X00881; X00882; X00883; X00884; X00885; X01237; X00741; M13872; M13873; M13874; AB021961; AF151353; AB017815; AB017816; AB020317; BC005448; S77930
Genbank Nucleotide ID CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25625.1; CAA25323.1; AAA39881.1; AAA39882.1; AAA39883.1; BAA82344.1; AAD39535.1; BAA82339.1; BAA82340.1; BAA82343.1; AAH05448.1; AAB21108.2
Protein Name Cellular tumor antigen p53
Protein Synonyms/Alias Tumor suppressor p53
Gene Name Tp53
Gene Synonyms/Alias P53
Trp53
Organism Mus musculus
NCBI Taxa ID 10090
Functional Description
(View all)
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Lysine Modification
PLMD PTMs: 12
PositionPeptidesTypeReferences ( PMIDs )
126TYSPPLNKFCQLAKAcetylation22826441
299ELPPGSAKALPTCTAcetylation22826441; 23806337
314SASPPQKKPLDGEYAcetylation16943427; 22826441
315ASPPQKKKLDGEYFAcetylation22826441; 23806337
325DGEYFTLKRGRKRFAcetylation23806337
364RAHSSYLKKKGQSTAcetylation22826441; 23806337
366HSSYLKTKGQSTSRAcetylation22826441
366HSSYLKTKGQSTSRMethylation25514926
367SSYLKTKKQSTSRHAcetylation22826441
375GQSTSRHKTMVKKVAcetylation22826441; 23806337
376QSTSRHKKMVKKVGAcetylation22826441; 23806337
380RHKKTMVKVGPDSDAcetylation22826441
Sequence
(Fasta)
MEESQSDISL ELPLSQETFS GLWKLLPPED ILPSPHCMDD LLLPQDVEEF FEGPSEALRV 60
SGAPAAQDPV TETPGPVAPA PATPWPLSSF VPSQKTYQGN YGFHLGFLQS GTAKSVMCTY 120
SPPLNKLFCQ LAKTCPVQLW VSATPPAGSR VRAMAIYKKS QHMTEVVRRC PHHERCSDGD 180
GLAPPQHLIR VEGNLYPEYL EDRQTFRHSV VVPYEPPEAG SEYTTIHYKY MCNSSCMGGM 240
NRRPILTIIT LEDSSGNLLG RDSFEVRVCA CPGRDRRTEE ENFRKKEVLC PELPPGSAKR 300
ALPTCTSASP PQKKKPLDGE YFTLKIRGRK RFEMFRELNE ALELKDAHAT EESGDSRAHS 360
SYLKTKKGQS TSRHKKTMVK KVGPDSD 387
Sequence Annotation CHAIN 1 387 Cellular tumor antigen p53.
DNA_BIND 96 286 {ECO:0000250}.
REGION 1 314 Interaction with CCAR2.
REGION 1 42 Transcription activation (acidic).
REGION 60 104 Interaction with WWOX. {ECO:0000250}.
REGION 94 364 Interaction with HIPK1.
REGION 94 294 Required for interaction with ZNF385A.
REGION 107 230 Required for interaction with FBXO42.
REGION 110 286 Interaction with AXIN1.
REGION 253 291 Interaction with E4F1. {ECO:0000250}.
REGION 267 274 Interaction with DNA.
REGION 313 354 Interaction with HIPK2. {ECO:0000250}.
REGION 319 350 Oligomerization.
REGION 353 357 Interaction with USP7. {ECO:0000250}.
REGION 362 381 Basic (repression of DNA-binding).
MOTIF 299 315 Bipartite nuclear localization signal.
MOTIF 333 344 Nuclear export signal. {ECO:0000250}.
MOTIF 364 366 [KR]-[STA]-K motif.
METAL 170 170 Zinc.
METAL 173 173 Zinc.
METAL 232 232 Zinc.
METAL 236 236 Zinc.
SITE 114 114 Interaction with DNA.
MOD_RES 9 9 Phosphoserine; by HIPK4.
MOD_RES 15 15 Phosphoserine; by CDK5, PRPK, AMPK, NUAK1
MOD_RES 18 18 Phosphothreonine; by CK1, VRK1 and VRK2.
MOD_RES 20 20 Phosphoserine; by CHEK2, CK1 and PLK3.
MOD_RES 34 34 Phosphoserine; by MAPKAPK5.
MOD_RES 114 114 N6-acetyllysine; by KAT6A. {ECO:0000250}.
MOD_RES 177 177 Phosphoserine; by AURKB. {ECO:0000250}.
MOD_RES 263 263 Phosphoserine; by AURKB. {ECO:0000250}.
MOD_RES 278 278 Phosphothreonine; by AURKB.
MOD_RES 299 299 N6-acetyllysine.
MOD_RES 309 309 Phosphoserine; by AURKA, CDK1 and CDK2.
MOD_RES 315 315 N6-acetyllysine.
MOD_RES 364 364 N6,N6-dimethyllysine; alternate.
MOD_RES 364 364 N6-methyllysine; by SMYD2; alternate.
MOD_RES 366 366 N6-methyllysine; by SETD7. {ECO:0000250}.
MOD_RES 367 367 N6,N6-dimethyllysine; by EHMT1 and EHMT2
;MOD_RES 367 367 N6-acetyllysine; alternate.
MOD_RES 375 375 N6-acetyllysine.
MOD_RES 376 376 N6,N6-dimethyllysine; alternate.
MOD_RES 376 376 N6-acetyllysine; by KAT6A; alternate.
MOD_RES 376 376 N6-methyllysine; by KMT5A; alternate.
MOD_RES 386 386 Phosphoserine; by CK2, CDK2 and NUAK1.
CROSSLNK 285 285 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 286 286 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 380 380 Glycyl lysine isopeptide (Lys-Gly)
CONFLICT 45 45 Q -> R (in Ref. 3; CAA25323).
CONFLICT 76 78 PVA -> QW (in Ref. 3; CAA25323).
HELIX 99 101 {ECO:0000244|PDB:2P52}.
STRAND 104 106 {ECO:0000244|PDB:2P52}.
HELIX 113 116 {ECO:0000244|PDB:3EXJ}.
STRAND 117 121 {ECO:0000244|PDB:2P52}.
TURN 122 125 {ECO:0000244|PDB:2P52}.
STRAND 126 129 {ECO:0000244|PDB:2P52}.
STRAND 134 140 {ECO:0000244|PDB:2P52}.
STRAND 150 159 {ECO:0000244|PDB:2P52}.
TURN 160 164 {ECO:0000244|PDB:2P52}.
HELIX 171 175 {ECO:0000244|PDB:2P52}.
STRAND 181 183 {ECO:0000244|PDB:2P52}.
STRAND 188 193 {ECO:0000244|PDB:2P52}.
STRAND 198 201 {ECO:0000244|PDB:2P52}.
TURN 203 205 {ECO:0000244|PDB:2P52}.
STRAND 208 213 {ECO:0000244|PDB:2P52}.
STRAND 222 230 {ECO:0000244|PDB:2P52}.
HELIX 235 237 {ECO:0000244|PDB:2P52}.
HELIX 239 242 {ECO:0000244|PDB:2P52}.
STRAND 245 252 {ECO:0000244|PDB:2P52}.
STRAND 258 268 {ECO:0000244|PDB:2P52}.
HELIX 272 283 {ECO:0000244|PDB:2P52}.
TURN 285 287 {ECO:0000244|PDB:3EXJ}.
Keyword

KW-0002--3D-structure.
KW-0007--Acetylation.
KW-0010--Activator.
KW-0053--Apoptosis.
KW-0090--Biological rhythms.
KW-0131--Cell cycle.
KW-0181--Complete proteome.
KW-0963--Cytoplasm.
KW-0225--Disease mutation.
KW-0238--DNA-binding.
KW-0256--Endoplasmic reticulum.
KW-1017--Isopeptide bond.
KW-0479--Metal-binding.
KW-0488--Methylation.
KW-0496--Mitochondrion.
KW-1210--Necrosis.
KW-0539--Nucleus.
KW-0597--Phosphoprotein.
KW-1185--Reference proteome.
KW-0678--Repressor.
KW-0804--Transcription.
KW-0805--Transcription regulation.
KW-0043--Tumor suppressor.
KW-0832--Ubl conjugation.
KW-0862--Zinc.

Interpro

IPR008967--p53-like_TF_DNA-bd.
IPR012346--p53/RUNT-type_TF_DNA-bd.
IPR011615--p53_DNA-bd.
IPR010991--p53_tetrameristn.
IPR013872--p53_transactivation_domain.
IPR002117--p53_tumour_suppressor.

PROSITE

PS00348--P53.

Pfam

PF00870--P53.
PF08563--P53_TAD.
PF07710--P53_tetramer.

Gene Ontology

GO:0000785--C:chromatin.
GO:0005737--C:cytoplasm.
GO:0005829--C:cytosol.
GO:0005783--C:endoplasmic reticulum.
GO:0005759--C:mitochondrial matrix.
GO:0005739--C:mitochondrion.
GO:0000790--C:nuclear chromatin.
GO:0016363--C:nuclear matrix.
GO:0005730--C:nucleolus.
GO:0005654--C:nucleoplasm.
GO:0005634--C:nucleus.
GO:0016605--C:PML body.
GO:0043234--C:protein complex.
GO:0005657--C:replication fork.
GO:0035861--C:site of double-strand break.
GO:0005667--C:transcription factor complex.
GO:0005524--F:ATP binding.
GO:0051087--F:chaperone binding.
GO:0003682--F:chromatin binding.
GO:0005507--F:copper ion binding.
GO:0001046--F:core promoter sequence-specific DNA binding.
GO:0003684--F:damaged DNA binding.
GO:0003677--F:DNA binding.
GO:0019899--F:enzyme binding.
GO:0035035--F:histone acetyltransferase binding.
GO:0035033--F:histone deacetylase regulator activity.
GO:0042802--F:identical protein binding.
GO:0097371--F:MDM2/MDM4 family protein binding.
GO:0002039--F:p53 binding.
GO:0002020--F:protease binding.
GO:0046982--F:protein heterodimerization activity.
GO:0019901--F:protein kinase binding.
GO:0047485--F:protein N-terminus binding.
GO:0051721--F:protein phosphatase 2A binding.
GO:0019903--F:protein phosphatase binding.
GO:0043621--F:protein self-association.
GO:0030971--F:receptor tyrosine kinase binding.
GO:0000979--F:RNA polymerase II core promoter sequence-specific DNA binding.
GO:0000977--F:RNA polymerase II regulatory region sequence-specific DNA binding.
GO:0000981--F:RNA polymerase II transcription factor activity, sequence-specific DNA binding.
GO:0001085--F:RNA polymerase II transcription factor binding.
GO:0003700--F:transcription factor activity, sequence-specific DNA binding.
GO:0008134--F:transcription factor binding.
GO:0044212--F:transcription regulatory region DNA binding.
GO:0001077--F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding.
GO:0001228--F:transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding.
GO:0031625--F:ubiquitin protein ligase binding.
GO:0006915--P:apoptotic process.
GO:0002326--P:B cell lineage commitment.
GO:0060411--P:cardiac septum morphogenesis.
GO:0007569--P:cell aging.
GO:0007050--P:cell cycle arrest.
GO:0006974--P:cellular response to DNA damage stimulus.
GO:0071480--P:cellular response to gamma radiation.
GO:0042149--P:cellular response to glucose starvation.
GO:0071479--P:cellular response to ionizing radiation.
GO:0034644--P:cellular response to UV.
GO:0071494--P:cellular response to UV-C.
GO:0007417--P:central nervous system development.
GO:0021549--P:cerebellum development.
GO:0031497--P:chromatin assembly.
GO:0031052--P:chromosome breakage.
GO:0051276--P:chromosome organization.
GO:0048512--P:circadian behavior.
GO:0007623--P:circadian rhythm.
GO:0008340--P:determination of adult lifespan.
GO:0030330--P:DNA damage response, signal transduction by p53 class mediator.
GO:0006977--P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest.
GO:0006978--P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator.
GO:0000733--P:DNA strand renaturation.
GO:0006302--P:double-strand break repair.
GO:0009792--P:embryo development ending in birth or egg hatching.
GO:0048568--P:embryonic organ development.
GO:0043153--P:entrainment of circadian clock by photoperiod.
GO:0006983--P:ER overload response.
GO:0007369--P:gastrulation.
GO:0007507--P:heart development.
GO:0001701--P:in utero embryonic development.
GO:0072332--P:intrinsic apoptotic signaling pathway by p53 class mediator.
GO:0042771--P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator.
GO:0070059--P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress.
GO:1990144--P:intrinsic apoptotic signaling pathway in response to hypoxia.
GO:0043504--P:mitochondrial DNA repair.
GO:0071850--P:mitotic cell cycle arrest.
GO:0031571--P:mitotic G1 DNA damage checkpoint.
GO:0007275--P:multicellular organism development.
GO:0035264--P:multicellular organism growth.
GO:0070266--P:necroptotic process.
GO:0043066--P:negative regulation of apoptotic process.
GO:0030308--P:negative regulation of cell growth.
GO:0008285--P:negative regulation of cell proliferation.
GO:0008156--P:negative regulation of DNA replication.
GO:0048147--P:negative regulation of fibroblast proliferation.
GO:0010629--P:negative regulation of gene expression.
GO:1904024--P:negative regulation of glucose catabolic process to lactate via pyruvate.
GO:1901525--P:negative regulation of macromitophagy.
GO:0045930--P:negative regulation of mitotic cell cycle.
GO:0007406--P:negative regulation of neuroblast proliferation.
GO:0045861--P:negative regulation of proteolysis.
GO:2000378--P:negative regulation of reactive oxygen species metabolic process.
GO:0051974--P:negative regulation of telomerase activity.
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter.
GO:0045892--P:negative regulation of transcription, DNA-templated.
GO:0030512--P:negative regulation of transforming growth factor beta receptor signaling pathway.
GO:0051402--P:neuron apoptotic process.
GO:0006289--P:nucleotide-excision repair.
GO:0097252--P:oligodendrocyte apoptotic process.
GO:0090403--P:oxidative stress-induced premature senescence.
GO:0043065--P:positive regulation of apoptotic process.
GO:0010666--P:positive regulation of cardiac muscle cell apoptotic process.
GO:0090343--P:positive regulation of cell aging.
GO:0071158--P:positive regulation of cell cycle arrest.
GO:1900119--P:positive regulation of execution phase of apoptosis.
GO:0010628--P:positive regulation of gene expression.
GO:0031065--P:positive regulation of histone deacetylation.
GO:2001244--P:positive regulation of intrinsic apoptotic signaling pathway.
GO:0035794--P:positive regulation of mitochondrial membrane permeability.
GO:0043525--P:positive regulation of neuron apoptotic process.
GO:0050731--P:positive regulation of peptidyl-tyrosine phosphorylation.
GO:0032461--P:positive regulation of protein oligomerization.
GO:2000379--P:positive regulation of reactive oxygen species metabolic process.
GO:0090200--P:positive regulation of release of cytochrome c from mitochondria.
GO:0070245--P:positive regulation of thymocyte apoptotic process.
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter.
GO:1990440--P:positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress.
GO:0061419--P:positive regulation of transcription from RNA polymerase II promoter in response to hypoxia.
GO:0045893--P:positive regulation of transcription, DNA-templated.
GO:0006461--P:protein complex assembly.
GO:0000060--P:protein import into nucleus, translocation.
GO:0008104--P:protein localization.
GO:0050821--P:protein stabilization.
GO:0051262--P:protein tetramerization.
GO:0042981--P:regulation of apoptotic process.
GO:0051726--P:regulation of cell cycle.
GO:0042127--P:regulation of cell proliferation.
GO:2000772--P:regulation of cellular senescence.
GO:2000269--P:regulation of fibroblast apoptotic process.
GO:0072363--P:regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter.
GO:1902253--P:regulation of intrinsic apoptotic signaling pathway by p53 class mediator.
GO:1902108--P:regulation of mitochondrial membrane permeability involved in apoptotic process.
GO:0043523--P:regulation of neuron apoptotic process.
GO:0070243--P:regulation of thymocyte apoptotic process.
GO:0034103--P:regulation of tissue remodeling.
GO:0006357--P:regulation of transcription from RNA polymerase II promoter.
GO:0006355--P:regulation of transcription, DNA-templated.
GO:0001836--P:release of cytochrome c from mitochondria.
GO:0090399--P:replicative senescence.
GO:0042493--P:response to drug.
GO:0010332--P:response to gamma radiation.
GO:0002931--P:response to ischemia.
GO:0006979--P:response to oxidative stress.
GO:0009651--P:response to salt stress.
GO:0009411--P:response to UV.
GO:0010165--P:response to X-ray.
GO:0009303--P:rRNA transcription.
GO:0001756--P:somitogenesis.
GO:0033077--P:T cell differentiation in thymus.
GO:0002360--P:T cell lineage commitment.
GO:0002309--P:T cell proliferation involved in immune response.
GO:0007179--P:transforming growth factor beta receptor signaling pathway.
GO:0016032--P:viral process.

KEGG Pathway mmu:22059