PLMD Protein Information


Tag Content
PLMD ID PLMD-0003220
Uniprot Accession P04637; Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98; Q3LRW1; Q3LRW2; Q3LRW3; Q3LRW4; Q3LRW5; Q86UG1; Q8J016; Q99659; Q9BTM4; Q9HAQ8; Q9NP68; Q9NPJ2; Q9NZD0; Q9UBI2; Q9UQ61
Genbank Protein ID X02469; M13121; M13112; M13113; M13114; M13115; M13116; M13117; M13118; M13119; M13120; K03199; M14694; M14695; M22898; M22882; M22883; M22884; M22887; M22888; M22894; M22895; M22896; M22897; X01405; X60011; X60012; X60013; X60014; X60015; X60016; X60017; X60018; X60019; X60020; AF307851; DQ186648; DQ186649; DQ186650; DQ186651; DQ186652; DQ191317; DQ286964; X54156; U94788; AY838896; AF135121; AF135120; AF136271; AF136270; AB082923; AK312568; AC007421; AC087388; CH471108; CH471108; BC003596; AY429684; AY390341; AY359814; U63714; AF209136; AF209128; AF209129; AF209130; AF209131; AF209132; AF209133; AF209134; AF209135; AF209148; AF209149; AF209150; AF209151; AF209152; AF209153; AF209154; AF209155; AF209156; AF210309; AF210308; AF210310; AF240684; AF240685; AY270155
Genbank Nucleotide ID CAA26306.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59989.1; AAA61211.1; AAA61212.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; CAA25652.1; CAA42626.1; CAA42627.1; CAA42628.1; CAA42629.1; CAA42630.1; CAA42631.1; CAA42632.1; CAA42633.1; CAA42634.1; CAA42635.1; AAG28785.1; ABA29753.1; ABA29754.1; ABA29755.1; ABA29756.1; ABA29757.1; ABB80262.1; ABB80266.1; CAA38095.1; AAC12971.1; AAV80424.1; AAD28535.1; AAD28535.1; AAD28628.1; AAD28628.1; BAC16799.1; BAG35463.1; EAW90143.1; EAW90144.1; AAH03596.1; AAR10356.1; AAQ90158.1; AAR13239.1; AAB39322.1; AAF36362.1; AAF36354.1; AAF36355.1; AAF36356.1; AAF36357.1; AAF36358.1; AAF36359.1; AAF36360.1; AAF36361.1; AAF36374.1; AAF36375.1; AAF36376.1
Protein Name Cellular tumor antigen p53
Protein Synonyms/Alias Antigen NY-CO-13
Phosphoprotein p53
Tumor suppressor p53
Gene Name TP53
Gene Synonyms/Alias P53
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View all)
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Lysine Modification
PLMD PTMs: 47 (View all)
PositionPeptidesTypeReferences ( PMIDs )
101SSSVPSQKYQGSYGUbiquitination21890473; 21906983; 23603789; 26051181
120FLHSGTAKVTCTYSAcetylation17189186; 17189187; 19308289
120FLHSGTAKVTCTYSUbiquitination21906983; 21963094; 21987572; 22053931; 26051181
132TYSPALNKFCQLAKUbiquitination21890473; 21963094; 23603789; 26051181
139KMFCQLAKCPVQLWUbiquitination21963094; 26051181
164VRAMAIYKSQHMTEAcetylation18485870
164VRAMAIYKSQHMTEUbiquitination21890473; 21963094; 23603789; 26051181
291TEEENLRKGEPHHEUbiquitination23603789
292EEENLRKKEPHHELAcetylation12724314; 19608861; 20167786
292EEENLRKKEPHHELPropionylation18753126
292EEENLRKKEPHHELUbiquitination21890473; 23603789
305ELPPGSTKALPNNTAcetylation12724314; 19155208; 19608861
305ELPPGSTKALPNNTUbiquitination21890473; 21906983; 21963094; 23603789; 26051181
319TSSSPQPKKPLDGEAcetylation17267393; 19155208
319TSSSPQPKKPLDGEButyrylation17267393
319TSSSPQPKKPLDGEPropionylation17267393
320SSSPQPKKPLDGEYAcetylation17267393; 19155208; 9744860; 9891054
320SSSPQPKKPLDGEYButyrylation17267393
320SSSPQPKKPLDGEYNeddylation17098746
320SSSPQPKKPLDGEYPropionylation17267393
Sequence
(Fasta)
MEEPQSDPSV EPPLSQETFS DLWKLLPENN VLSPLPSQAM DDLMLSPDDI EQWFTEDPGP 60
DEAPRMPEAA PPVAPAPAAP TPAAPAPAPS WPLSSSVPSQ KTYQGSYGFR LGFLHSGTAK 120
SVTCTYSPAL NKMFCQLAKT CPVQLWVDST PPPGTRVRAM AIYKQSQHMT EVVRRCPHHE 180
RCSDSDGLAP PQHLIRVEGN LRVEYLDDRN TFRHSVVVPY EPPEVGSDCT TIHYNYMCNS 240
SCMGGMNRRP ILTIITLEDS SGNLLGRNSF EVRVCACPGR DRRTEEENLR KKGEPHHELP 300
PGSTKRALPN NTSSSPQPKK KPLDGEYFTL QIRGRERFEM FRELNEALEL KDAQAGKEPG 360
GSRAHSSHLK SKKGQSTSRH KKLMFKTEGP DSD 393
Sequence Annotation CHAIN 1 393 Cellular tumor antigen p53.
DNA_BIND 102 292
REGION 1 320 Interaction with CCAR2.
REGION 1 83 Interaction with HRMT1L2.
REGION 1 44 Transcription activation (acidic).
REGION 66 110 Interaction with WWOX.
REGION 100 370 Interaction with HIPK1. {ECO:0000250}.
REGION 100 300 Required for interaction with ZNF385A.
REGION 113 236 Required for interaction with FBXO42.
REGION 116 292 Interaction with AXIN1. {ECO:0000250}.
REGION 241 248 Interaction with the 53BP2 SH3 domain.
REGION 256 294 Interaction with E4F1.
REGION 273 280 Interaction with DNA.
REGION 300 393 Interaction with CARM1.
REGION 319 360 Interaction with HIPK2.
REGION 325 356 Oligomerization.
REGION 359 363 Interaction with USP7.
REGION 368 387 Basic (repression of DNA-binding).
MOTIF 17 25 TADI.
MOTIF 48 56 TADII.
MOTIF 305 321 Bipartite nuclear localization signal.
MOTIF 339 350 Nuclear export signal.
MOTIF 370 372 [KR]-[STA]-K motif.
METAL 176 176 Zinc.
METAL 179 179 Zinc.
METAL 238 238 Zinc.
METAL 242 242 Zinc.
SITE 120 120 Interaction with DNA.
MOD_RES 9 9 Phosphoserine; by HIPK4.
MOD_RES 15 15 Phosphoserine; by CDK5, PRPK, AMPK, NUAK1
MOD_RES 18 18 Phosphothreonine; by CK1, VRK1 and VRK2.
MOD_RES 20 20 Phosphoserine; by CHEK2, CK1 and PLK3.
MOD_RES 33 33 Phosphoserine; by CDK5 and CDK7.
MOD_RES 37 37 Phosphoserine; by MAPKAPK5.
MOD_RES 46 46 Phosphoserine; by CDK5, DYRK2, HIPK2 and
MOD_RES 55 55 Phosphothreonine; by TAF1 and GRK5.
MOD_RES 120 120 N6-acetyllysine; by KAT6A.
MOD_RES 183 183 Phosphoserine; by AURKB.
MOD_RES 269 269 Phosphoserine; by AURKB.
MOD_RES 284 284 Phosphothreonine; by AURKB.
MOD_RES 305 305 N6-acetyllysine.
MOD_RES 315 315 Phosphoserine; by AURKA, CDK1 and CDK2.
MOD_RES 321 321 N6-acetyllysine.
MOD_RES 370 370 N6,N6-dimethyllysine; alternate.
MOD_RES 370 370 N6-methyllysine; by SMYD2; alternate.
MOD_RES 372 372 N6-methyllysine; by SETD7.
MOD_RES 373 373 N6,N6-dimethyllysine; by EHMT1 and EHMT2
;MOD_RES 373 373 N6-acetyllysine; alternate.
MOD_RES 381 381 N6-acetyllysine.
MOD_RES 382 382 N6,N6-dimethyllysine; alternate.
MOD_RES 382 382 N6-acetyllysine; by KAT6A; alternate.
MOD_RES 382 382 N6-methyllysine; by KMT5A; alternate.
MOD_RES 392 392 Phosphoserine; by CK2, CDK2 and NUAK1.
CROSSLNK 291 291 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 292 292 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 386 386 Glycyl lysine isopeptide (Lys-Gly)
VAR_SEQ 1 132 Missing (in isoform 7, isoform 8 and
VAR_SEQ 1 39 Missing (in isoform 4, isoform 5 and
VAR_SEQ 332 346 IRGRERFEMFRELNE -> MLLDLRWCYFLINSS (in
VAR_SEQ 332 341 IRGRERFEMF -> DQTSFQKENC (in isoform 2,
VAR_SEQ 342 393 Missing (in isoform 2, isoform 5 and
VAR_SEQ 347 393 Missing (in isoform 3, isoform 6 and
MUTAGEN 15 15 S->A: Loss of interaction with PPP2R5C,
MUTAGEN 18 18 T->A: No effect on interaction with MDM2
MUTAGEN 20 20 S->A: Abolishes phosphorylation site.
MUTAGEN 20 20 S->D: Constitutively increased TP53
MUTAGEN 22 23 LW->QS: Loss of interaction with MDM2,
MUTAGEN 37 37 S->D: Abolihes phosphorylation by
MUTAGEN 46 46 S->A: Abolishes phosphorylation by DYRK2
MUTAGEN 46 46 Missing: Alters interaction with WWOX.
MUTAGEN 55 55 T->A: Blocks phosphorylation by TAF1.
MUTAGEN 183 183 S->A: Abolishes strongly phosphorylation.
MUTAGEN 183 183 S->E: Inhibits slightly its
MUTAGEN 248 248 R->S: Does not induce SNAI1 degradation.
MUTAGEN 269 269 S->A: Abolishes phosphorylation.
MUTAGEN 269 269 S->E: Inhibits strongly its
MUTAGEN 284 284 T->E: Inhibits strongly its
MUTAGEN 291 292 KK->RR: Abolishes polyubiquitination by
MUTAGEN 319 319 K->A: Loss of nuclear localization; when
MUTAGEN 320 320 K->A: Loss of nuclear localization; when
MUTAGEN 321 321 K->A: Loss of nuclear localization; when
MUTAGEN 359 359 P->D: Abolishes binding to USP7.
MUTAGEN 361 361 G->E: Abolishes binding to USP7.
MUTAGEN 362 362 S->A: Abolishes binding to USP7.
MUTAGEN 370 370 K->R: Induces a decrease in methylation
MUTAGEN 372 372 K->R: Induces a decrease in protein
MUTAGEN 373 373 K->R: Abolishes dimethylation by EHMT1
MUTAGEN 382 382 K->A: Abolishes acetylation by CREBBP.
MUTAGEN 382 382 K->R: Abolishes monomethylation by KMT5A.
MUTAGEN 383 383 L->A: Abolishes S-315 phosphorylation by
MUTAGEN 385 385 F->A: Reduced SUMO1 conjugation.
MUTAGEN 386 386 K->A: Abolishes SUMO1 conjugation, in
MUTAGEN 387 387 T->A: No effect SUMO1 conjugation.
MUTAGEN 388 388 E->A: Abolishes SUMO1 conjugation.
HELIX 3 6 {ECO:0000244|PDB:5HOU}.
TURN 8 10 {ECO:0000244|PDB:5HOU}.
HELIX 19 23 {ECO:0000244|PDB:3DAC}.
STRAND 27 29 {ECO:0000244|PDB:2K8F}.
HELIX 30 32 {ECO:0000244|PDB:5HPD}.
STRAND 33 35 {ECO:0000244|PDB:2L14}.
HELIX 36 38 {ECO:0000244|PDB:2B3G}.
HELIX 41 44 {ECO:0000244|PDB:2B3G}.
HELIX 47 55 {ECO:0000244|PDB:2B3G}.
TURN 105 108 {ECO:0000244|PDB:3D06}.
STRAND 110 112 {ECO:0000244|PDB:3D06}.
STRAND 119 121 {ECO:0000244|PDB:5BUA}.
STRAND 124 127 {ECO:0000244|PDB:3D06}.
TURN 128 131 {ECO:0000244|PDB:3D06}.
STRAND 132 135 {ECO:0000244|PDB:3D06}.
STRAND 141 146 {ECO:0000244|PDB:3D06}.
STRAND 156 165 {ECO:0000244|PDB:3D06}.
HELIX 166 168 {ECO:0000244|PDB:3D06}.
HELIX 177 180 {ECO:0000244|PDB:3D06}.
STRAND 181 183 {ECO:0000244|PDB:4KVP}.
STRAND 187 189 {ECO:0000244|PDB:4MZI}.
STRAND 194 199 {ECO:0000244|PDB:3D06}.
STRAND 204 207 {ECO:0000244|PDB:3D06}.
TURN 209 211 {ECO:0000244|PDB:3D06}.
STRAND 214 219 {ECO:0000244|PDB:3D06}.
TURN 225 227 {ECO:0000244|PDB:2FEJ}.
STRAND 228 236 {ECO:0000244|PDB:3D06}.
HELIX 240 242 {ECO:0000244|PDB:3D07}.
TURN 243 248 {ECO:0000244|PDB:3D06}.
STRAND 251 258 {ECO:0000244|PDB:3D06}.
STRAND 260 262 {ECO:0000244|PDB:2ADY}.
STRAND 264 274 {ECO:0000244|PDB:3D06}.
HELIX 278 287 {ECO:0000244|PDB:3D06}.
HELIX 288 290 {ECO:0000244|PDB:4HJE}.
HELIX 322 324 {ECO:0000244|PDB:3Q01}.
STRAND 327 334 {ECO:0000244|PDB:3Q01}.
HELIX 335 354 {ECO:0000244|PDB:1AIE}.
HELIX 375 380 {ECO:0000244|PDB:1YC5}.
HELIX 381 385 {ECO:0000244|PDB:4X34}.
Keyword

KW-0002--3D-structure.
KW-0007--Acetylation.
KW-0010--Activator.
KW-0877--Alternative promoter usage.
KW-0025--Alternative splicing.
KW-0053--Apoptosis.
KW-0090--Biological rhythms.
KW-0131--Cell cycle.
KW-0181--Complete proteome.
KW-0963--Cytoplasm.
KW-0225--Disease mutation.
KW-0238--DNA-binding.
KW-0256--Endoplasmic reticulum.
KW-0325--Glycoprotein.
KW-0945--Host-virus interaction.
KW-1017--Isopeptide bond.
KW-0435--Li-Fraumeni syndrome.
KW-0479--Metal-binding.
KW-0488--Methylation.
KW-0496--Mitochondrion.
KW-1210--Necrosis.
KW-0539--Nucleus.
KW-0597--Phosphoprotein.
KW-0621--Polymorphism.
KW-1185--Reference proteome.
KW-0678--Repressor.
KW-0804--Transcription.
KW-0805--Transcription regulation.
KW-0043--Tumor suppressor.
KW-0832--Ubl conjugation.
KW-0862--Zinc.

Interpro

IPR008967--p53-like_TF_DNA-bd.
IPR012346--p53/RUNT-type_TF_DNA-bd.
IPR011615--p53_DNA-bd.
IPR010991--p53_tetrameristn.
IPR013872--p53_transactivation_domain.
IPR002117--p53_tumour_suppressor.

PROSITE

PS00348--P53.

Pfam

PF00870--P53.
PF08563--P53_TAD.
PF07710--P53_tetramer.

Gene Ontology

GO:0005737--C:cytoplasm.
GO:0005829--C:cytosol.
GO:0005783--C:endoplasmic reticulum.
GO:0005759--C:mitochondrial matrix.
GO:0005739--C:mitochondrion.
GO:0000790--C:nuclear chromatin.
GO:0016363--C:nuclear matrix.
GO:0005730--C:nucleolus.
GO:0005654--C:nucleoplasm.
GO:0005634--C:nucleus.
GO:0016605--C:PML body.
GO:0043234--C:protein complex.
GO:0005657--C:replication fork.
GO:0005524--F:ATP binding.
GO:0051087--F:chaperone binding.
GO:0003682--F:chromatin binding.
GO:0005507--F:copper ion binding.
GO:0001046--F:core promoter sequence-specific DNA binding.
GO:0003684--F:damaged DNA binding.
GO:0003677--F:DNA binding.
GO:0003690--F:double-stranded DNA binding.
GO:0019899--F:enzyme binding.
GO:0035035--F:histone acetyltransferase binding.
GO:0042802--F:identical protein binding.
GO:0002039--F:p53 binding.
GO:0002020--F:protease binding.
GO:0046982--F:protein heterodimerization activity.
GO:0019901--F:protein kinase binding.
GO:0047485--F:protein N-terminus binding.
GO:0051721--F:protein phosphatase 2A binding.
GO:0019903--F:protein phosphatase binding.
GO:0043621--F:protein self-association.
GO:0030971--F:receptor tyrosine kinase binding.
GO:0000977--F:RNA polymerase II regulatory region sequence-specific DNA binding.
GO:0000981--F:RNA polymerase II transcription factor activity, sequence-specific DNA binding.
GO:0001085--F:RNA polymerase II transcription factor binding.
GO:0043565--F:sequence-specific DNA binding.
GO:0003700--F:transcription factor activity, sequence-specific DNA binding.
GO:0008134--F:transcription factor binding.
GO:0044212--F:transcription regulatory region DNA binding.
GO:0001228--F:transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding.
GO:0031625--F:ubiquitin protein ligase binding.
GO:0008270--F:zinc ion binding.
GO:0006284--P:base-excision repair.
GO:0007569--P:cell aging.
GO:0007050--P:cell cycle arrest.
GO:0030154--P:cell differentiation.
GO:0008283--P:cell proliferation.
GO:0034613--P:cellular protein localization.
GO:0006974--P:cellular response to DNA damage stimulus.
GO:0035690--P:cellular response to drug.
GO:0042149--P:cellular response to glucose starvation.
GO:0071456--P:cellular response to hypoxia.
GO:0071479--P:cellular response to ionizing radiation.
GO:0034644--P:cellular response to UV.
GO:0031497--P:chromatin assembly.
GO:0048512--P:circadian behavior.
GO:0008340--P:determination of adult lifespan.
GO:0030330--P:DNA damage response, signal transduction by p53 class mediator.
GO:0006977--P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest.
GO:0006978--P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator.
GO:0000733--P:DNA strand renaturation.
GO:0043153--P:entrainment of circadian clock by photoperiod.
GO:0006983--P:ER overload response.
GO:0097193--P:intrinsic apoptotic signaling pathway.
GO:0072332--P:intrinsic apoptotic signaling pathway by p53 class mediator.
GO:0042771--P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator.
GO:0031571--P:mitotic G1 DNA damage checkpoint.
GO:0007275--P:multicellular organism development.
GO:0043066--P:negative regulation of apoptotic process.
GO:0030308--P:negative regulation of cell growth.
GO:0008285--P:negative regulation of cell proliferation.
GO:0048147--P:negative regulation of fibroblast proliferation.
GO:0051097--P:negative regulation of helicase activity.
GO:0051974--P:negative regulation of telomerase activity.
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter.
GO:0045892--P:negative regulation of transcription, DNA-templated.
GO:0006289--P:nucleotide-excision repair.
GO:0097252--P:oligodendrocyte apoptotic process.
GO:0090403--P:oxidative stress-induced premature senescence.
GO:0043065--P:positive regulation of apoptotic process.
GO:0071158--P:positive regulation of cell cycle arrest.
GO:1900119--P:positive regulation of execution phase of apoptosis.
GO:0010628--P:positive regulation of gene expression.
GO:0031065--P:positive regulation of histone deacetylation.
GO:2001244--P:positive regulation of intrinsic apoptotic signaling pathway.
GO:0043525--P:positive regulation of neuron apoptotic process.
GO:0050731--P:positive regulation of peptidyl-tyrosine phosphorylation.
GO:0046827--P:positive regulation of protein export from nucleus.
GO:1900740--P:positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway.
GO:0032461--P:positive regulation of protein oligomerization.
GO:2000379--P:positive regulation of reactive oxygen species metabolic process.
GO:0090200--P:positive regulation of release of cytochrome c from mitochondria.
GO:0070245--P:positive regulation of thymocyte apoptotic process.
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter.
GO:1990440--P:positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress.
GO:0045893--P:positive regulation of transcription, DNA-templated.
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process.
GO:0006461--P:protein complex assembly.
GO:0008104--P:protein localization.
GO:0016925--P:protein sumoylation.
GO:0051262--P:protein tetramerization.
GO:0007265--P:Ras protein signal transduction.
GO:0042981--P:regulation of apoptotic process.
GO:1902749--P:regulation of cell cycle G2/M phase transition.
GO:0046902--P:regulation of mitochondrial membrane permeability.
GO:1901796--P:regulation of signal transduction by p53 class mediator.
GO:0006355--P:regulation of transcription, DNA-templated.
GO:0090399--P:replicative senescence.
GO:0046677--P:response to antibiotic.
GO:0010332--P:response to gamma radiation.
GO:0010165--P:response to X-ray.
GO:0016032--P:viral process.

KEGG Pathway hsa:7157