PLMD Protein Information


Tag Content
PLMD ID PLMD-0034014
Uniprot Accession Q96S44; B3KU44; Q3T977; Q5JZ01; Q6NZ60; Q96FM7; Q9NQE6
Genbank Protein ID AB017505; AB065434; AK096502; AL031055; AL133520; AL133520; AL031055; CH471077; BC009727; BC010637; BC066309
Genbank Nucleotide ID BAB61875.2; BAB62041.1; BAG53306.1; CAC00561.1; CAC00561.1; CAJ30016.1; CAJ30016.1; EAW75727.1; AAH09727.1; AAH10637.1; AAH66309.1
Protein Name TP53-regulating kinase
Protein Synonyms/Alias Atypical serine/threonine protein kinase TP53RK
EKC/KEOPS complex subunit TP53RK
Nori-2
p53-related protein kinase
Gene Name TP53RK
Gene Synonyms/Alias C20orf64
PRPK
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View all)
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Lysine Modification
PLMD PTMs: 4
PositionPeptidesTypeReferences ( PMIDs )
134QSTMETEKPQGLSNUbiquitination21906983; 21963094
144QGLSNLAKIGQVLAUbiquitination23266961
238KARPVLKKDEVRLRUbiquitination24816145
40LSGLELVKGAEARVUbiquitination24816145
Sequence
(Fasta)
MAAARATTPA DGEEPAPEAE ALAAARERSS RFLSGLELVK QGAEARVFRG RFQGRAAVIK 60
HRFPKGYRHP ALEARLGRRR TVQEARALLR CRRAGISAPV VFFVDYASNC LYMEEIEGSV 120
TVRDYIQSTM ETEKTPQGLS NLAKTIGQVL ARMHDEDLIH GDLTTSNMLL KPPLEQLNIV 180
LIDFGLSFIS ALPEDKGVDL YVLEKAFLST HPNTETVFEA FLKSYSTSSK KARPVLKKLD 240
EVRLRGRKRS MVG 253
Sequence Annotation CHAIN 1 253 TP53-regulating kinase.
DOMAIN 33 253 Protein kinase. {ECO:0000255|PROSITE-
NP_BIND 39 47 ATP. {ECO:0000255|PROSITE-
MOTIF 78 95 Nuclear localization signal.
ACT_SITE 162 162 Proton acceptor. {ECO:0000255|PROSITE-
BINDING 60 60 ATP. {ECO:0000255|PROSITE-
MOD_RES 34 34 Phosphoserine.
MOD_RES 135 135 Phosphothreonine.
CONFLICT 51 51 R -> H (in Ref. 6; AAH66309).
CONFLICT 93 93 R -> L (in Ref. 6; AAH10637).
Keyword

KW-0067--ATP-binding.
KW-0181--Complete proteome.
KW-0378--Hydrolase.
KW-0418--Kinase.
KW-0547--Nucleotide-binding.
KW-0539--Nucleus.
KW-0597--Phosphoprotein.
KW-0621--Polymorphism.
KW-1185--Reference proteome.
KW-0723--Serine/threonine-protein kinase.
KW-0808--Transferase.
KW-0819--tRNA processing.

Interpro

IPR022495--Bud32.
IPR011009--Kinase-like_dom.
IPR010440--LipoPS_kinase.
IPR000719--Prot_kinase_dom.
IPR008266--Tyr_kinase_AS.

PROSITE

PS50011--PROTEIN_KINASE_DOM.
PS00109--PROTEIN_KINASE_TYR.

Pfam

PF06293--Kdo.

Gene Ontology

GO:0005829--C:cytosol.
GO:0000408--C:EKC/KEOPS complex.
GO:0016020--C:membrane.
GO:0005654--C:nucleoplasm.
GO:0005634--C:nucleus.
GO:0005524--F:ATP binding.
GO:0016787--F:hydrolase activity.
GO:0002039--F:p53 binding.
GO:0004674--F:protein serine/threonine kinase activity.
GO:0006468--P:protein phosphorylation.
GO:1901796--P:regulation of signal transduction by p53 class mediator.
GO:0008033--P:tRNA processing.
GO:0070525--P:tRNA threonylcarbamoyladenosine metabolic process.

KEGG Pathway hsa:112858